Coding

Part:BBa_K2365510

Designed by: yuanqi jia   Group: iGEM17_NAU-CHINA   (2017-10-22)


Homo sapiens estrogen1

The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. ERα plays a role in the physiological development and function of a variety of organ systems to varying degrees, including the reproductive, central nervous, skeletal, and cardiovascular systems. Accordingly, ERα is widely expressed throughout the body, including the uterus and ovary, male reproductive organs, mammary gland, bone, heart, hypothalamus, pituitary gland, liver, lung, kidney, spleen, and adipose tissue. In our project we utilize ER to specifically bind ZEN for our detecting purpose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 193
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 430
    Illegal NgoMIV site found at 438
    Illegal NgoMIV site found at 719
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1168
    Illegal SapI.rc site found at 702


[edit]
Categories
Parameters
None